Fluorescent Staining Method for Bacterial Endospores

نویسندگان

  • Denise Schichnes
  • Jeffery A. Nemson
  • Steven E. Ruzin
چکیده

We have developed a quick, simple technique for the fluorescent staining of bacterial endospores. This technique was developed using Bacillus subtilis endospores. Our technique requires minimal sample heating so it can safely be carried out entirely in a glove-box. The endospores stain diagnostically as an oval with a standard fluorescent nucleotide stain, acridine orange.

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تاریخ انتشار 2007